In vitro callus induction from a highly medicinal plant: Pterocarpus marsupium Roxb

The current study has focused on an efficient in vitro callus induction protocol for the Pterocarpus marsupium Roxb. plant. Explants from healthy plants, including leaves and stem nodes, were employed to induce callus formation. Murashige and Skoog medium supplemented with 2,4D resulted in the highest callus proliferation. Callus induction varies based on the type of explant and growth hormone concentration employed. Massive callus was achieved at 1.0 mg/L of 2, 4 D and BAP each utilizing a leaf explant.