IgE, IgA, IgD antibodies detection in human cysticercosis by ELISA test in Madagascar

RAMANDANIRAINY, Prisca Annick and RAMAROSON, Vololoniaina Roseline and RAZAFIARIMANGA, Zara and RAZAFIMAHEFA, Julien and JAMBOU, Ronan and ANDRIANARANJAKA, Voahangy and SIMAHAVANDY, Abel ANDRIAN (2025) IgE, IgA, IgD antibodies detection in human cysticercosis by ELISA test in Madagascar. World Journal of Advanced Research and Reviews, 27 (2). pp. 1591-1597. ISSN 2581-9615

Cysticercosis serological diagnosis available in Madagascar is only based on the IgG detection. Thus, we have planned to perform biological tests in order to detect different human antibodies isotypes (IgE, IgA, IgD) directed against Cysticercus cellulosae and to evaluate the performance of enzyme-linked immunosorbent assay (ELISA). CS-50 antigen, extracted from Cysticercus cellulosae was used to quantify the rates of these isotypes in the serum and in the cerebrospinal fluid (CSF). After standardizing protocols, 80 patients with neurocysticercosis symptoms from the Neurology Department, Joseph Raseta Hospital Befelatanana, Antananarivo were analyzed. Results showed high rate of IgD in the serum and high rate of IgA in the CSF. For each studied isotype Ig, ELISA test performance was low compared to ELISA-IgG test as well in the serum than in the CSF.