Research on genes identification for antiseptic action in some basil genotypes

Chiuoaru, Mădălin Vasile and Chira, Adrian and Nagy, Daniel and Butnariu, Gabriela and Ion, Ligia (2025) Research on genes identification for antiseptic action in some basil genotypes. World Journal of Advanced Research and Reviews, 27 (2). pp. 1266-1274. ISSN 2581-9615

The studies undertaken by the present work refer to 21 domestic and foreign genotypes, in which a screening was done with 8 molecular markers, for the investigation of the genome in terms of highlighting the gene that controls the antiseptic action and the resistance of plants to biotic factors. The collection of basil genotypes is not very large in Romania. There are only 150 autochthonous basil genotypes. The genomic DNA isolated from the basil genotypes, “Aromat de Buzau”, “BZ 1”, “Hof Igal 2”, “Gea”, “LBRS 2”, “LBVS 1”, etc. was used to implement of RADP molecular markers to highlight the gene that controls antiseptic action. In order to determine, the DNA samples extracted from young leaves by the methods of Lodhi et al. 1994, modified by Pop et al., 2003 and Doyle & Doyle, 1990, were quantified/measured, amplified, and the amplification products (RAPDs) were electrophoretic ally separated in agarose gel. The research aimed to highlight the ObGAPDH gene in basil genotypes taken in the study using 8 specifically designed molecular markers.