In Silico Rational Design and Immunoinformatic Analysis of a Multi-Epitope Peptide Vaccine Targeting Neuroinflammatory Pathways in Migraine

Pigili, Akhil Kumar and Kanemela, Chandra Kumar and Guntamadugu, Srinivas and katika, Anil Kumar Babu and Yashwanth, kommu (2025) In Silico Rational Design and Immunoinformatic Analysis of a Multi-Epitope Peptide Vaccine Targeting Neuroinflammatory Pathways in Migraine. World Journal of Biology Pharmacy and Health Sciences, 23 (2). pp. 161-175. ISSN 2582-5542

Migraine is a major neurovascular disorder affecting millions of people globally with the current therapies which are not limited to the long-term prevention. The chronic Neuroinflammation which is a symptom of migraine. This involves pathways which are linked by the CGRP, PACAP, and TRPV which are linked to the Neuroinflammation and pain signalling. Our study aims the development of the multi epitope based peptide vaccine candidates which are protein driven primarily targeting the CGRP as the primary target. As cgrp is the key target protein in the migraine management. In this study we have targeted the key target proteins and analysed their physiochemical properties and epitopes were screened and screened epitopes were identified with the toxicity, allergenicity, antigenicity, followed by the vaccine constructs using the linkers and adjuvants. The tertiary structures were identified and the molecular docking was performed with the tlr3, tlr4, and tlr6. The final vaccine construct which was screened exhibited the high immunogenicity, stability, and non-allergenicity is observed.