Spectroscopic method development and validation for simultaneous estimation of neomycin sulphate and clotrimazole from bulk and pharmaceutical dosage form

Swami, Vijaya U. and Ali, Syed Shoaib and Pawar, Komal K. and Jain, Chetan M. (2025) Spectroscopic method development and validation for simultaneous estimation of neomycin sulphate and clotrimazole from bulk and pharmaceutical dosage form. GSC Biological and Pharmaceutical Sciences, 32 (1). 017-024. ISSN 2581-3250

A variety of pharmacological medications are available for the treatment of fungal infections. Spectroscopic methods are very effective for estimating such drugs. The study aimed to develop and validate a simple, sensitive, rapid, and precise UV-visible method for simultaneously estimating neomycin sulphate and clotrimazole in bulk and pharmaceutical dosage form. The λmax of neomycin sulphate and clotrimazole were found to be 244 nm and 263nm. The drug exhibits linearity within the range of 300-1800 ug/ml for neomycin sulphate and 2-12 ug/ml for clotrimazole. The minimal % RSD values indicate the method's accuracy and precision. The robustness of this method was examined by varying its wavelength. Method was found to be sensitive based on LOD and LOQ values of both drugs. It was evident from the results that the suggested method works well for the quick, precise, and accurate measurement of Neomycin sulphate and Clotrimazole by UV-Visible spectroscopy method.