Method development and Validation of Catechin hydrate by using UV-Visible Spectroscopy

Pawar, Komal K. and Ali, Syed Shoaib and Jain, Chetan M. and Khavane, Karna B. (2025) Method development and Validation of Catechin hydrate by using UV-Visible Spectroscopy. GSC Biological and Pharmaceutical Sciences, 32 (1). 001-006. ISSN 2581-3250

Catechin hydrate, a polyphenolic molecule, is widely present in tea leaves, fruits, and vegetables. The present study aims to develop an innovative, uncomplicated, precise, and reliable UV-visible spectroscopic technique to estimate Catechin Hydrate. The λmax of catechin hydrate in methanol: water (90:10) was 278 nm. The drug exhibits linearity within the range of 2–12 μg/ml, with a R2 value of 0.9991. The accuracy of selected method was assessed using a recovery study at three distinct levels: 80%, 100%, and 120%. The % recovery was determined to be between 99.83% and 101.08%. The minimal % RSD values indicate the method's accuracy and repeatability. The precision study was examined using intraday and interday changes. A % RSD value of less than 2 indicates the procedure's precision. The robustness of the suggested approach was examined by varying its wavelength. The LOD and LOQ were found to be 0.314 and 1.148 µg/ml, respectively. The results clearly show that the UV-visible spectroscopic method may effectively be used to estimate the concentration of Catechin hydrate.