The potential of red betel leaf as a Hypoglycemic Agent in Alloxan-Induced Mice

Johari, Asni and Mailuddillahi, Viona and Muswita, Muswita and Naswir, Muhammad (2025) The potential of red betel leaf as a Hypoglycemic Agent in Alloxan-Induced Mice. Open Access Research Journal of Biology and Pharmacy, 13 (2). 001-006. ISSN 2782-9979

This study aims to determine the effect of red betel leaf extract and its optimal dose in reducing blood glucose levels in alloxan-induced male mice. This experimental study employs a Completely Randomized Design with a quantitative approach, using 6 treatments with 5 replications. The alloxan dose administered is 150 mg/kg body weight (BW), while the red betel leaf extract doses are 50, 100, 150, and 200 mg/kg BW. Glibenclamide is used as a positive control at a dose of 0.65 mg/kg BW, administered via the mouse’s vein. Blood samples were collected using a syringe and measured using a glucometer 5 times. Data analysis was conducted using Analysis of Variance (ANOVA), and if a significant effect was observed, it was followed by Duncan's Multiple Range Test at an α = 5% significance level. Based on the ANOVA analysis of the blood glucose reduction percentage, red betel leaf extract demonstrated an effect in lowering blood glucose levels. Duncan's Multiple Range Test indicated that treatment P3 (150 mg/kg BW) showed a significant difference compared to other treatments, with the highest percentage reduction in blood glucose level of 41.06%. Thus, the optimal dose of red betel leaf extract for lowering blood glucose levels in mice is 150 mg/kg BW. The results of this study are expected to serve as a source of information for developing alternative treatments for diabetes mellitus in humans.