Detection Salmonella sp. from swab clooca of broiler chicken at Keputran market, Surabaya Indonesia

Bestari, Nissa Maulia and Witaningrum, Adiana Mutamsari and Rahadrjo, Dadik and Hendrawan, Viski Fitri and Widjiati, Widjiati and Luqman, Epy Muhammad and Tyasningsih, Wiwiek (2025) Detection Salmonella sp. from swab clooca of broiler chicken at Keputran market, Surabaya Indonesia. Open Access Research Journal of Biology and Pharmacy, 13 (1). 021-029. ISSN 2782-9979

This study aims to determine the presence of Salmonella sp. from broiler cloacal swabs at Keputran Market, Surabaya Indonesia. The research methods used include sampling, isolation and identification using Tetrathionate Broth Base (TTB) Media, Salmonella Shigella Agar (SSA) Media and followed by Biochemical Test. Inoculation of samples on Tetrathionate Broth Base (TTB) Media followed by inoculation on Salmonella Shigella Agar (SSA) Media produces a transparent colony and there is a black spot in the middle. Microscopic examination was done by Gram staining. Suspected colony of Salmonella sp. were then purified and identify by biochemical tests. Bacterial biochemical test media used, such as Triple Sugar Iron Agar (TSIA), Simon Citrate Agar (SCA), Sulfide Indole Motility (SIM), Urea Test and Carbohydrate fermentation test. The results of the study from 30 samples of broiler cloacal swabs at Keputran Market, Surabaya found 2 positive samples detected of Salmonella sp.