Verification of the analytical performance of the serum anti-thyroperoxidase antibody assay on the Abbott Alinity ci®: Experience from the Biochemistry Laboratory of the Mohammed VI University Hospital of Oujda

Jamal, Kaoutar and Kharkhach, Oumaima and Rchid, Sara Moulay and Douichi, Imane and Moujtahide, Dounia El and Sebbar, El-Houcine and Choukri, Mohammed (2025) Verification of the analytical performance of the serum anti-thyroperoxidase antibody assay on the Abbott Alinity ci®: Experience from the Biochemistry Laboratory of the Mohammed VI University Hospital of Oujda. GSC Biological and Pharmaceutical Sciences, 30 (2). pp. 261-268. ISSN 2581-3250

The objective of this study was to evaluate the analytical performance of the measurement of anti-thyroperoxidase antibodies (anti-TPO antibodies) in human serum and plasma, using chemiluminescent microparticle immunoassay (CMIA), in accordance with Domain A criteria of the medical biology method verification/validation guide. The results of the anti-TPO antibody serum assays show good repeatability for the three levels (1: low, 2: medium, and 3: high), with respective coefficients of variation (CV) of 1.67%, 1.97%, and 1.17%. Similarly, intermediate precision is considered satisfactory, with CV of 1.90% for level 1, 1.96% for level 2, and 1.38% for level 3. Comparison with the reference CV defined by the RICOS (Reference Institute for Clinical and Healthcare Standards) confirms the reliability and compliance of the results. The results allowed us to verify the performance of the light chain Lambda assay method and compare it with the analytical objectives set in the accreditation process in which our laboratory is engaged.