Reliability of PSA assays: A key asset for early prostate cancer detection

Rhoubi, Asmae and Faiz, Ismail and Joudar, fatima zahra and Sebbar, El Houcine and Moujtahid, Dounia El and Choukri, Mohamed (2025) Reliability of PSA assays: A key asset for early prostate cancer detection. GSC Biological and Pharmaceutical Sciences, 30 (2). 001-006. ISSN 2581-3250

Introduction: Prostate cancer requires early detection to reduce mortality, with PSA serving as a key marker since 1987. This assay, crucial for distinguishing benign conditions from cancer, requires rigorous precision in the laboratory. The aim of this study was to verify the method of PSA measurement in the biochemistry laboratory of the CHU Mohammed VI d'Oujda. Materials and methods: This prospective study, conducted at the CHU Mohammed VI d'Oujda over 30 days, assessed the reliability of total and free PSA measurements on the Abbott Alinity ci® analyzer. It assessed reproducibility through multi-operator tests and repeatability through consecutive analyses of standardized samples. The CMIA method enabled rigorous quantitative measurement, with analysis of coefficients of variation compared to CLSI standards to guarantee precision and accuracy, validated by intermediate management software. Results: The study assessed the repeatability and reproducibility of total and free PSA assays at different concentration levels. The results indicate that the majority of values fall within the acceptable range of variation ([-1S, 1S]), although minor variations were observed depending on levels and handling conditions. These results confirm the accuracy of the assay methods and underline their reliability for robust clinical analyses. Discussion and conclusion: Our results demonstrate good repeatability and reproducibility of tPSA and fPSA assays on Abbott's Alinity ci® analyzer, in line with performance standards established in the literature.