Effect of β-Carotene patch application on micronucleus formation of gingival epithelial cells exposed to panoramic radiography, an in vivo study

Shantiningsih, Rurie Ratna and Mudjosemedi, Munakhir and Astuti, Eha Renwi (2025) Effect of β-Carotene patch application on micronucleus formation of gingival epithelial cells exposed to panoramic radiography, an in vivo study. World Journal of Advanced Research and Reviews, 27 (1). 090-094. ISSN 2581-9615

Objective: Previous studies have indicated that the number of micronucleus, a marker of DNA damage, is increased in gingival mucosa after a panoramic examination. β-Carotene is the lipid-soluble antioxidant substance and expected to prevent the oxidative reaction by improves the junctional communication and reduce the risk from DNA damage. The objective of this study was to monitor the changes of micronucleus during panoramic examination by applying β-Carotene Patch to rabbit gingiva. Methods: A total of 18 New Zealand rabbits were divided into 2 groups equally. Group, I was the control group that was given a panoramic examination without applying β-Carotene Patch. Group II was the test group that applied β-Carotene Patch before the panoramic examination. Micronucleus samples were taken from rabbit’s gingival mucosa then stained by the method of modified Feulgen-Rosenbeck. The results were analyzed using one-way ANOVA (P < 0.05). Results: The number of micronucleus in Group I was significantly higher than those in the Group II (P = 0.001). Conclusion: β-Carotene Patch may reduce the number of micronucleus formation of rabbit’s gingival epithelial cells exposed to panoramic radiography.