Investigation of antimitotic activity in ethanolic leaf extract of herbals: Euphorbia milii

Sushma, D and Bikkannagari, Harivardhan and Vadlamanu, Priyanka and Jellala, Pranitha and Hanumadri, Nithin and Kulla, Manga (2025) Investigation of antimitotic activity in ethanolic leaf extract of herbals: Euphorbia milii. World Journal of Biology Pharmacy and Health Sciences, 23 (1). pp. 312-322. ISSN 2582-5542

Humans are using plants as medicine around from 6000 years and most of the drugs in modern science are plant derived or plant extracts. As per the science we have investigated antimitotic activity in a plant extract. The antimitotic activity of ethanolic extract of Euphorbia milii leaves was studied by using seed germination assay. The antimitotic drugs which are formerly developed has the phytochemicals such as alkaloids, flavonoids and saponins. As the phytochemical study of Euphorbia milii proved that it has phytochemicals such as carbohydrates, alkaloids, tannins, saponins and phenolic compounds. The ethanolic extract of Euphorbia milii leaves has shown a significant amount of antimitotic activity in the seed germination assay. When compared to the methotrexate (0.1 mg/ml), the 40 mg/ml (p<0.0001) of the ethanolic extract of Euphorbia milii leaves at different doses (10 mg/ml, 20 mg/ml, and 40 mg/ml) shown an exceptional effect or significance. Thus, ethanolic leaf extract of Euphorbia milii shows antimitotic activity and can be used in the treatment of cancer. Further evaluations should be done in the future.