Synergistic effect of grape seed extract in combination with green coffee bean for the treatment of diabetes mellitus

VELLAISAMY, JAWAHAR and SOLAIYAPPAN, SOLAIJAYALAKSHMI (2025) Synergistic effect of grape seed extract in combination with green coffee bean for the treatment of diabetes mellitus. World Journal of Biology Pharmacy and Health Sciences, 23 (1). pp. 272-278. ISSN 2582-5542

The proposed project successful attempt has been made to synergistic effect of grape seed extract in combination with green coffee bean for the treatment of diabetes mellitus. I started process plant extraction is a modern technique that offers a rapid method for extraction phytoconstituents. Coffee arabica extract percentage yield 11.44% and grape seed extract percentage yield 10.23% mixture of these two extract were taken 1:1 ratio for the present study to find the synergistic anti diabetic activity phytochemical study conducted to investigate the present various components CBGSC and process of Alpha-Amylase inhibition activity extract lower that the standard acarbose[IC50=9.08µg/ml]grape seed extract was used alone AAI rate 24.20ug/ml and green coffee extract AAI-rate 20.13µg/ml combination of extract observed at 92.56%at360ug/ml IC50 was found to be 17.33µg/ml. alpha-glucosidase inhibition GS extract concentration 88.72% AGE.IC50 value 17.04µg/ml. GC Extract concentration 89.61%AGE.IC50 value 15.42µg/ml. Molecular docking analysis was conducted human alpha glucosidase binding energy and beta -D-glucopyranosyl binding energy 9.39Kcal/mol . and inhibition constant 130.88nm. Binding site of ligand hydroxyl group of Ligand -binding distance LLN167o-H-o -3.2, ASP199O-H-o-3.1, ASG411N-H-o-2.6 All the process are followed by as per WHO and ICH guidelines