Antioxidant activity of a Polyherbal formulation ‘ACHF-01’ by DPPH radical scavenging assay

Chavan, Sumedh Sunil and Shekade, Pratiksha Prabhakar and Dhabe, Arvind Shankarao (2025) Antioxidant activity of a Polyherbal formulation ‘ACHF-01’ by DPPH radical scavenging assay. World Journal of Advanced Research and Reviews, 26 (3). pp. 1199-1202. ISSN 2581-9615

This study evaluates the antioxidant potential of an herbal formulation through the 2, 2-dipheny l – 1- picrylhydrazyl (DPPH) free radical scavenging assay. Polyherbal formulation ‘ACHF-01’ was tested, with ascorbic acid serving as the positive control. The percentage inhibition of DPPH radicals of sample ‘ACHF-01’ demonstrating the 55.78% activity. These findings highlight the potential of specific herbal combinations as natural antioxidants and suggest the need for further phytochemical analysis and In vivo validation.