Pharmacognostical, Phytochemical Evaluation of Kokilakshakam Kashayam: A classical herbal formulation

Kuvine, P. and Joy, Anu and Sandhya, V.R and Francis, Smitha and Ramanathan, D (2025) Pharmacognostical, Phytochemical Evaluation of Kokilakshakam Kashayam: A classical herbal formulation. World Journal of Biology Pharmacy and Health Sciences, 21 (3). pp. 588-594. ISSN 2582-5542

Ayurveda, a holistic healing system, uses medicinal formulations like Kokilakshakam Kashayam to treat conditions such as Vatarakta (gout), characterized by excessive uric acid accumulation in joints. This Ayurvedic decoction combines Hygrophila auriculata (Kokilaksha) and Tinospora cordifolia (Guduchi), both known for their anti-inflammatory, detoxifying, and rejuvenating properties. Despite its traditional use, scientific documentation of its pharmacognostic, phytochemical, and pharmacological profiles is limited. This study aimed to evaluate the raw materials, preparation process, and therapeutic properties of Kokilakshakam Kashayam. The raw materials were sourced from Sitaram Ayurveda Pvt. Ltd. and authenticated through pharmacognostic analysis. The formulation was prepared according to traditional methods outlined in Sarangadhara Samhita. Physicochemical analysis of the Kashayam showed parameters like pH, specific gravity and total soluble solids. Preliminary phytochemical tests revealed bioactive compounds including terpenoids, flavonoids, alkaloids, and saponins. Thin Layer Chromatography (TLC) fingerprinting confirmed the presence of several therapeutic compounds. These findings support the traditional use of Kokilakshakam Kashayam for its anti-inflammatory, antioxidant, and antimicrobial effects, suggesting its potential to reduce inflammation, improve metabolic functions, and promote overall health. This study provides scientific validation for Kokilakshakam Kashayam, contributing to its standardization as a therapeutic option for managing gout and related inflammatory conditions. Further clinical research is recommended to explore its long-term efficacy and integration into modern medicine.