Dose-dependent effects of vitamin C on oxidative stress and kidney function in Albino Rats

Ifenkwe, John Chimerenka and Ononuju, Uzochukwu Alozie (2025) Dose-dependent effects of vitamin C on oxidative stress and kidney function in Albino Rats. World Journal of Biology Pharmacy and Health Sciences, 21 (2). pp. 471-475. ISSN 2582-5542

Vitamin C is an essential nutrient that plays a crucial role in various body functions. While it is generally considered safe, high-dose administration has raised concerns about potential toxicological effects. This study aimed to investigate the toxicological effects of high-dose vitamin C administration in animal models. 80 male albino rats which were divided into four groups: Control, Low-dose (100 mg/kg), Medium-dose (500 mg/kg), and High-dose (1000 mg/kg) vitamin C administration were used for the study. Vitamin C was administered orally once daily for 28 days. Oxidative stress and kidney damage were assessed using standard biochemical assays. 4-Hydroxynonenal (4-HNE) levels were measured to assess oxidative stress while urea and creatinine levels were measured to assess the possible effects on the kidneys. Our results show that high-dose vitamin C administration caused significant increases in oxidative stress and kidney dysfunction in rats. These findings suggest that high-dose vitamin C administration may have adverse effects on health, and its use should be approached with caution.