Hepatoprotective Activity of Oak methanolic extract on paracetamol induced Albino Male Mice

Ismael, Shahad Basil and Al-ezzy, Ruqaya Mohammed (2025) Hepatoprotective Activity of Oak methanolic extract on paracetamol induced Albino Male Mice. International Journal of Science and Research Archive, 15 (2). pp. 1820-1828. ISSN 2582-8185

Background: The liver, the main metabolic organ, monitors the toxicity of substances, including medications. When avoiding complications from damaging the liver, protection is crucial. Objectives: This research aimed to Detection of Quercus robur active constituent as well as determination of liver function enzyme in mic blood serum such as aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) after treated with paracetamol, Determination of total protein urea and creatin concentration in blood serum after treated with paracetamol and Histological evaluation of albino male mice liver after different treatment. Methods: The hepatoprotective evaluations included assessment of liver function enzyme (aspartate aminotransferase; AST and alanine aminotransferase; ALT and alkaline phosphate; ALP) in serum, as well as histopathological evaluation of liver tissue, in mice after the administration with paracetamol. Results: The results showed the extract possessed hepatoprotective ability against paracetamol liver damage by their ability to counteract paracetamol effect on AST, ALT and ALP and histological liver section. The results of AST in serum of mice were (70 ± 1.4 U\L) for negative control groups, while the level of AST enzyme (38 ± 1.1 U\L) for Oak (Quercus)treated group, for paracetamol treated group, the enzyme level was (40 ± 2.3 U\L) for drug group, and finally for interaction groups (36 ± 2.6 U\L) for Oak + paracetamol. The results of ALT were (29 ± 2.6, 23 ± 3.7, 21 ± 4.2 and 16 ± 1.1U\L) for negative control group, Quercus treated group, paracetamol treated group, and interaction group respectively. Finally, the level of ALP was (243 ± 2.8, 201 ± 2.8, 215 ± 3.5 and178 ± 0.8 U\L) for negative control, Quercus treated group, paracetamol treated group, and interaction group respectively. The level of Creatinine were (0.09 ± 0.003, 0.11 ± 0.002, 0.07 ± 0.001 and 0.09 ± 0.003 MG\DL) for negative control, Quercus treated group, paracetamol treated group, and interaction group respectively The level of Total Protein were (55 ± 0.12, 53 ± 0.14, 53 ± 0.12 and 55 ± 0.19 G\L ) for negative control, Quercus treated group, paracetamol treated group, and interaction group respectively Finally, the level of Urea were (30 ± 0.01, 40 ± 0.02, 20 ± 0.001 and 30 ± 0.02 MG\DL ) for negative control, Quercus treated group, paracetamol treated group, and interaction group respectively. Conclusion: Our results suggest secondary metabolite presented in plant such as (Saponins, Polyphenols, Alkaloids and Glycosides), that possessed hepatoprotective activity.