Administration of hydroxyapatite and ellagic acid combination to bone defects on Interleukin-6 Expression

Nirwana, Intan and Yuliati, Anita and Setiawan, Harits Al-Farizi and Bermani, Ahmad Thariq and Shahaniz, Yasmin (2025) Administration of hydroxyapatite and ellagic acid combination to bone defects on Interleukin-6 Expression. World Journal of Advanced Research and Reviews, 26 (1). pp. 1028-1034. ISSN 2581-9615

Background: Bone defect is the loss of some bone tissue which can be caused by several factors, namely trauma, infection, tumor, or congenital abnormalities. Efforts to repair bone defects can be done by giving bone grafts. This study used a xenograft derived from bovine bone, namely hydroxyapatite. Hydroxyapatite combined with Ellagic acid which is a polyphenol has antioxidant and anti-inflammatory properties which are expected to reduce Interleukin-6. Interleukin-6 is a marker that indicates the presence of an inflammatory process. Objective: To analyze the administration of a combination of Hydroxyapatite and Ellagic Acid to bone defects on the expression of IL-6. Methods: This study was a randomized post test only with control group design. The research sample used 30 Wistar rats with a defect in the left femur, and were divided into 3 groups, namely group K- with PEG, P1 with PEG and HA, and P2 with PEG, HA, and EA. On the 7th and 14th days, femoral bone tissue was taken to observe IL-6 expression using immunohistochemical techniques. Observations were made under a light microscope with a magnification of 400x. Results: Statistical analysis showed there was a significant difference between groups (p<0.05). Conclusion: Administration of a combination of HA and EA to bone defects of Wistar rats can reduce the expression of IL-6 for bone regeneration in the span of 7 and 14 days.