CADD studies on evaluation of cruciferous vegetable components on anti-oxidant activity via Nrf2 pathway

Apoorva, S. and Veeresh, B. and Vanamamalai, Sai Prathyusha and Likitha, M and Salagondla, Santhosh Rao and Pathakala, Naveen (2025) CADD studies on evaluation of cruciferous vegetable components on anti-oxidant activity via Nrf2 pathway. International Journal of Science and Research Archive, 15 (1). pp. 1417-1425. ISSN 2582-8185

The current study directed to progress antioxidant activity of cruciferous vegetable components on adaptor proteins and peroxiredoxins. Nrf2 is a transcription factor (basic leucine zipper protein). Under basal conditions it is located in the cytosol and under unfavourable conditions it translocates into the nucleus and aids in transcriptional activation of cytoprotective genes. Nrf2 plays a crucial role in determining the sensitivity of cells to oxidative stress. Some of the receptor proteins such as 7k2f, 7k2j, 7k2m, 7kiz, 7kj0 has undergone In-silico evaluation with chemical constituents, Sulforphane (Standard) and Glucosinolates (ligand) which are abundantly present in Cruciferous vegetables. To evaluate the receptor-ligand interaction between Brassicaceae chemical constituents and receptor proteins in the activation of Nrf2 pathway; Chemical components of Cruciferous vegetables were constructed by Chemsketch (2022 1.2) and they were subjected to molecular docking using Autodock Vina tools with the respective proteins. The ligand have greater binding affinity with the proteins when compared with Sulforphane and that concluded, Glucosinolates has more interaction towards the proteins when compared to Sulforphane. The results clearly revealed that Cruciferous components have enhanced antioxidant activity in the activation of Nrf2 pathway under extreme oxidative stress conditions.