Effect of ethanol extract of ginger (Zingiber officinale) on the histology of ciprofloxacin-induced testes of male Wistar rats

Ukwa, Agbai Johnson and Ifegwu, Njoku Oji and Mbanaso, Eberechukwu Lolly and Akataobi, Kelechi Uzoma and Agim, Cosmas Sopuruchi (2025) Effect of ethanol extract of ginger (Zingiber officinale) on the histology of ciprofloxacin-induced testes of male Wistar rats. World Journal of Advanced Research and Reviews, 25 (3). pp. 1799-1806. ISSN 2581-9615

Objective: This research study was carried out to investigate the effect of ethanol extract of ginger (Zingiber officinale) on the histology of Ciprofloxacin-induced testes of male wistar rats. Methodology: Twenty-five (25) male wistar rats weighing 110 g to 150 g were procured and acclimatized for two weeks, after which, they were divided into five (5) groups of five (5) rats each, and were housed in cages. The groups were designated as groups A - E. Group A served as the control group and was not induced with Ciprofloxacin, while Groups B – E were induced. Group A received distilled water only, Groups B - E received Ciprofloxacin only, Vitamin C, 100 mg/kg of body weight of ethanol extract of Zingiber officinale, and 300 mg/kg of body weight of ethanol extract of Zingiber officinale respectively for 14 days through oral route with the aid of oral gastric tube. On the 15th day, the animals were weighed and sacrificed via chloroform inhalation, and testes were harvested from the rats for histological study. Results: Histopathological findings showed a testicular tissue with active seminiferous tubules that are lined with interstitial cells of the Leydig (ICL), Sertoli cell (SC), and moderately enhanced spermatogenesis (S) for animals in group A; severe degeneration with severe spermatogenic arrest (SA), severe interstitial fibrosis (IF), and severe necrotic (N) appearance of the testicular cells for animals in group B; adequate healing with moderately enhanced spermatogenesis (S) and moderate active appearance of the testicular cells for animals in group C; mild regeneration with moderate spermatogenic arrest (SA), severe interstitial fibrosis (IF) and moderate necrotic (N) appearance of the testicular cells for animals in group D; and mild regeneration with moderate spermatogenic arrest (SA), and mild necrotic (N) appearance of the testicular cells for animals in group E. Conclusion: Ethanol extracts of Zingiber officinale have ameliorating effect on the histology of Ciprofloxacin-induced testes of male wistar rats, and the ameliorating effect improves with increase in the dosages of the extract