High-performance liquid chromatography (HPLC): Innovations in analytical techniques and applications

Ansari, Abdul Wadood and Yadav, Abhishek and Yadav, Ashish Kumar and Yadav, Manish and Arti, Arti (2025) High-performance liquid chromatography (HPLC): Innovations in analytical techniques and applications. International Journal of Science and Research Archive, 15 (1). pp. 155-166. ISSN 2582-8185

High-Performance Liquid Chromatography (HPLC) is a type of column chromatography that is commonly used in biochemistry and analysis to separate, identify, and quantify active chemicals. HPLC is the most often used separation technology for detecting, separating, and quantifying the drug High -Performance Liquid Chromatography (HPLC): A Comprehensive Review of Its, Principle, Detectors, And Application It works on the principle of Affinity chromatography having two phases viz: stationary and mobile phase. The constituent with lower affinity for stationary phase travels faster and vice-versa. HPLC is just one type of liquid chromatography, meaning the mobile phase is a liquid. Reversed-phase HPLC is the most common type of HPLC. The reversed-phase means the mobile phase is relatively polar, and the stationary phase is relatively non-polar. HPLC instrumentation includes a Solvent reservoir, pump, injector, column, detector, and integrator or acquisition and display system This article was written with the intention of reviewing several HPLC-related topics, including its principle, kinds, manner of separation, characteristics, instrumentation, key parameters, and numerous applications in various sectors.